Skip to main content
BIOINFORMATICS-BASICS5 MIN READ

Read a DESeq2 result row

Interpret a differential expression result row without overclaiming biological certainty.

Interpret this DESeq2 row for treated versus control: baseMean=84, log2FoldChange=1.4, lfcSE=0.38, pvalue=0.0008, padj=0.03 in a model ~ batch + condition. A differential expression row combines effect size, uncertainty, and multiple-testing-adjusted evidence for one modeled contrast. Saying "turned on" turns a statistical association in RNA counts into a mechanism claim the table does not prove. State the contrast The row describes treated versus control after accounting for batch in the design. Without the contrast and design, the direction and adjustment context can be misunderstood. Translate log2FC log2FC 1.4 means treated is estimated at about 2^1.4, or roughly 2.6-fold higher expression than…

Read the full lesson

Sign up free — one personalized lesson every day, matched to your role and goals.

Already have an account? Sign in

← Back to library
Contact us