FASTQ and QC at your fingertips
Recall core FASTQ and QC concepts used in first-pass sequencing review.
FASTQ What are the four lines of one FASTQ read? Think structure before biology. Identifier, sequence, plus line, and quality string. The sequence and quality string should correspond base by base. Quality score Q What does Phred Q summarize? Error probability on a log scale. Common objection The report has adapter content. Does that mean the sample failed? Adapter sequence appears in a subset of short-read libraries. Your response It means adapter sequence is present in reads. The first move is adapter trimming and post-trim QC; sample failure depends on how much usable sequence remains. Calling the sample failed before…
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