BIOINFORMATICS-BASICS5 MIN READ
Ask for replicates before the run is locked
Explain replication and confounding risks in a way that improves study design before sequencing.
Dr. Vale DV Principal investigator C Claim M Model D Design Before sequencing 20 sec Pilot design The budget allows a small RNA-seq pilot, but the proposed layout confounds treatment with prep day. A design problem now becomes an interpretation problem later. 1 replicate per group proposed 2 prep days tied to condition
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